Journal: Oncotarget

Article Title: Expression analysis and clinical significance of eIF4E, VEGF-C, E-cadherin and MMP-2 in colorectal adenocarcinoma

doi: 10.18632/oncotarget.13453

Figure Lengend Snippet: A. The expression of eIF4E in normal colon tissues around cancer. B. The negative expression of eIF4E in colon cancerous tissues. C. The positive expression of eIF4E in colon cancerous tissues. D. The expression of VEGF-C in normal colon tissues around cancer. E. The negative expression of VEGF-C in colon cancerous tissues. F. The positive expression of VEGF-C in colon cancerous tissues. G. The expression of E-cadherin (E-cad) in normal colon tissues around cancer. H. The negative expression of E-cad in colon cancerous tissues. I. The positive expression of E-cad in colon cancerous tissues. J. The expression of MMP-2 in normal colon tissues around cancer. K. The negative expression of MMP-2 in colon cancerous tissues. L. The positive expression of MMP-2 in colon cancerous tissues. Bar = 50 μM.

Article Snippet: eIF4E cDNA was inserted into the eukaryotic retroviral expression vector pBabe. eIF4E and control siRNAs were purchased from Ribobio (Cat No Q000001977-1-B, Guangzhou, People's Republic of China).

Techniques: Expressing

Journal: Oncotarget

Article Title: Expression analysis and clinical significance of eIF4E, VEGF-C, E-cadherin and MMP-2 in colorectal adenocarcinoma

doi: 10.18632/oncotarget.13453

Figure Lengend Snippet: Relationship between the expression of eIF4E, VEGF-C, E-cadherin and MMP-2 as well as clinical pathological parameters

Article Snippet: eIF4E cDNA was inserted into the eukaryotic retroviral expression vector pBabe. eIF4E and control siRNAs were purchased from Ribobio (Cat No Q000001977-1-B, Guangzhou, People's Republic of China).

Techniques: Expressing

Journal: Oncotarget

Article Title: Expression analysis and clinical significance of eIF4E, VEGF-C, E-cadherin and MMP-2 in colorectal adenocarcinoma

doi: 10.18632/oncotarget.13453

Figure Lengend Snippet: Log rank test χ2= 75.0054. p< 0.0001. Fragments of human colon tumors were implanted subcutaneously (s.c.) into nude mice, and mice with tumors that reached 4–5 mm in diameter (n=5 per group) were applied to survival statistics. A. Kaplan–Meier survival curves of mice bearing eIF4E negative and positive tumors. B. Kaplan–Meier survival curves of mice bearing VEGF-C negative and positive tumors. C. Kaplan–Meier survival curves of mice bearing MMP-2 negative and positive tumors. D. Kaplan–Meier survival curves of mice bearing E-cadherin negative and positive tumors.

Article Snippet: eIF4E cDNA was inserted into the eukaryotic retroviral expression vector pBabe. eIF4E and control siRNAs were purchased from Ribobio (Cat No Q000001977-1-B, Guangzhou, People's Republic of China).

Techniques:

Journal: Oncotarget

Article Title: Expression analysis and clinical significance of eIF4E, VEGF-C, E-cadherin and MMP-2 in colorectal adenocarcinoma

doi: 10.18632/oncotarget.13453

Figure Lengend Snippet: A. The negative expression of eIF4E in colon cancerous tissues. B. The positive expression of eIF4E in colon cancerous tissues. C. The negative expression of VEGF-C in colon cancerous tissues. D. The positive expression of VEGF-C in colon cancerous tissues. E. The negative expression of E-cadherin in colon cancerous tissues. F. The positive expression of E-cadherin in colon cancerous tissues. G. The negative expression of MMP-2 in colon cancerous tissues. H. The positive expression of MMP-2 in colon cancerous tissues. Bar = 25 μM.

Article Snippet: eIF4E cDNA was inserted into the eukaryotic retroviral expression vector pBabe. eIF4E and control siRNAs were purchased from Ribobio (Cat No Q000001977-1-B, Guangzhou, People's Republic of China).

Techniques: Expressing

Journal: Oncotarget

Article Title: Expression analysis and clinical significance of eIF4E, VEGF-C, E-cadherin and MMP-2 in colorectal adenocarcinoma

doi: 10.18632/oncotarget.13453

Figure Lengend Snippet: A. Western blotting showed that the stable cell lines including HCT-15/Rluc/E-cadherin, HCT-15/Rluc/VEGF-C, HCT-15/Rluc/MMP-2, and HCT-15/Rluc/eIF4E were constructed successfully. B. The stable colon cancer cell lines with overexpression of E-cadherin, MMP-2, VEGF-c and eIF4E as well as parental HCT-15/Rluc cell line showed different growth rates.

Article Snippet: eIF4E cDNA was inserted into the eukaryotic retroviral expression vector pBabe. eIF4E and control siRNAs were purchased from Ribobio (Cat No Q000001977-1-B, Guangzhou, People's Republic of China).

Techniques: Western Blot, Stable Transfection, Construct, Over Expression

Journal: Oncotarget

Article Title: Expression analysis and clinical significance of eIF4E, VEGF-C, E-cadherin and MMP-2 in colorectal adenocarcinoma

doi: 10.18632/oncotarget.13453

Figure Lengend Snippet: A. The HCT-15/Rluc/eIF4E cells (1.0 × 10 6 ) and corresponding HCT-15-Rluc were injected via tail-vein 3 days later. The bioluminescence seen represents the thorax region of the mouse where HCT-15/Rluc and HCT-15/Rluc/eIF4E cells are trapped in the lungs. B. The HCT-15/Rluc/VEGF-C cells (1.0 × 10 6 ) and corresponding HCT-15/Rluc were injected via tail-vein 3 days later. The bioluminescence seen represents the thorax region of the mouse where HCT-15/Rluc and HCT-15/Rluc/VEGF-C cells are trapped in the lungs. C. The HCT-15/Rluc/MMP-2 cells (1.0 × 10 6 ) and corresponding HCT-15/Rluc were injected via tail-vein 3 days later. The bioluminescence seen represents the thorax region of the mouse where HCT-15/Rluc and HCT-15/Rluc/MMP-2 cells are trapped in the lungs. D. The HCT/15-Rluc/E-cadherin cells (1.0 × 10 6 ) and corresponding HCT-15-Rluc were injected via tail-vein 3 days later. The bioluminescence seen represents the thorax region of the mouse where HCT-15/Rluc and HCT-15/Rluc/E-cadherin cells are trapped in the lungs.

Article Snippet: eIF4E cDNA was inserted into the eukaryotic retroviral expression vector pBabe. eIF4E and control siRNAs were purchased from Ribobio (Cat No Q000001977-1-B, Guangzhou, People's Republic of China).

Techniques: Injection

Journal: Oncotarget

Article Title: Expression analysis and clinical significance of eIF4E, VEGF-C, E-cadherin and MMP-2 in colorectal adenocarcinoma

doi: 10.18632/oncotarget.13453

Figure Lengend Snippet: A. Western blotting showed the expression of eIF4E, VEGF-C, MMP-2, and E-cadherin in the corresponding stable SW 480 cell lines with the overexpression and knockdown of eIF4E. Control, Control stable SW480 cell lines by use of lentiviral infection packaged with control empty vectors. Overexpression, the stable SW480 cell line with the overexpression of eIF4E. sheIF4E, the stable SW480 cell lines with knockdown of eIF4E. The cells were lysed for loading on SDS-PAGE. The blotting were performed by use of indicated antibodies. B. Q-PCR showed the expression of eIF4E, VEGF-C, MMP-2, and E-cadherin in the stable SW 480 cell lines with the overexpression of eIF4E. C. Q-PCR showed the expression of eIF4E, VEGF-C, MMP-2, and E-cadherin in the stable SW 480 cell lines with the knockdown of eIF4E. Control, Control stable SW480 cell lines by use of lentiviral infection packaged with control empty vectors. Overexpression of eIF4E, the stable SW480 cell line with the overexpression of eIF4E. sheIF4E, the stable SW480 cell lines with knockdown of eIF4E. *, p<0.05. D. Relative number of migrating cells ( y -axis) in a transwell migration assay is shown for Control, eIF4E-overexpression, and eIF4E-knockdown SW 480 cells. *, p<0.05. E. Number of mammospheres ( y -axis) formed by Control, eIF4E-overexpression and eIF4E-knockdown SW480 cells. *, p<0.05.

Article Snippet: eIF4E cDNA was inserted into the eukaryotic retroviral expression vector pBabe. eIF4E and control siRNAs were purchased from Ribobio (Cat No Q000001977-1-B, Guangzhou, People's Republic of China).

Techniques: Western Blot, Expressing, Over Expression, Knockdown, Control, Infection, SDS Page, Transwell Migration Assay